>100 Gbp on 1 flowcell outside ONT

After unboxing the Nanopore alpha-beta PromethION device some weeks ago and having our IT support/linux ninja help us integrate yet another box into the university network, we finally got around to run some PromethION sequencing again. We updated the PromethION software to Minknow 2 inserted a flowcell and it passed QC with 7000+ pores available […]

What is a good genome assembly?

With short reads you will often get fragmented but high single base accuracy assemblies  and with long error prone reads you can get a single contig assembly with just a few ultra long reads but with a lot of errors due to the lower read quality. Some of these errors can be fixed by adding more coverage […]

Promethion configuration test and “reboxing”

Following our PromethION unboxing event we have finally managed to connect the machine to our University network and pass the configuration run. The configuration test demonstrates that the network can handle the data produced and transfer it fast enough to a network storage solution.   Got everything up and running for @nanopore #PromethION CTC run thx to […]

Promethion unboxing

Following our experiences with DNA sequencing using the MinION since 2014 as a part of the Minion (early) Access Programme (MAP), and their developers programme we applied for a spot on the PromethION Early Access Programme (PEAP) back in May 2015. The MinION was the mindblowing DNA sequencer that allows you to do long read (no […]