Why is it important to remove short molecules?

In the age of short read sequencing you could treat the cells with very harsh physical disruption as there was little chance that your DNA extraction could shear your DNA to shorter fragments than the machines could sequence. However, with the emerging long read sequencers short fragments are suddenly no fun and people have started […]

Article recap: method biases in microbial community profilling of drinking water with 16S rRNA gene amplicon sequencing

In recent years, 16S rRNA gene amplicon sequencing has been widely adopted for analyzing microbial communities in drinking water. This has naturally lead to numerous publications relating to the drinking water microbiome. However, microbial analysis based on 16S rRNA gene sequencing is associated with inherent biases, and despite the popularity of the method within the field […]

>100 Gbp on 1 flowcell outside ONT

After unboxing the Nanopore alpha-beta PromethION device some weeks ago and having our IT support/linux ninja help us integrate yet another box into the university network, we finally got around to run some PromethION sequencing again. We updated the PromethION software to Minknow 2 inserted a flowcell and it passed QC with 7000+ pores available […]

The Roblon Prize: my first prize

Each year, the Roblon foundation awards the Roblon Prize of 100,000 DKK (~16,500 USD), to a master thesis from Aalborg University acclaimed to fulfill the following: “The thesis should be of highest quality and innovative. It should have a positive effect on the research field as well as the future research and career of the […]

ampvis2: A guide to ordination and how to use amp_ordinate in R

(This post is part of a series of ongoing posts about selected ampvis2 features) A common way for microbial ecologists to compare the microbial communities of different samples is by using ordination methods. These methods seek to highlight differences between samples based on their microbial community composition. By plotting samples as points in an x/y-plane, […]

NCM17 – Day 2: Into the unknown

Day 2 at the NCM17 started, again, with Oxford Nanopore CEO Gordon Sanghera taking the stage, once again stating his dream that sequencing should be available to anybody, anytime, anywhere. There are currently around 12,500 mainframe DNA sequencers around the globe, a number he believes will be passed by ONT in a not too distant future. […]

NCM17 – Day 1: New tech and exciting applications

All the gear We start out with blazing punk rock (things are never too boring at Oxford Nanopore meetings) and plenty of promises from Oxford Nanopore CEO Gordon Sanghera. He gave us updates on various products, such as PromethION flow cells, which have been harder to produce than expected. However, we and others have now sequenced […]