Since November we have run 11 PromethION flow cells, generating >900 Gbp of data. It has been a journey of highs and lows, so we want to share what we have learned along the way. Our first lesson: it is important to use those flow cells ASAP! Despite our lab’s earlier success with a three […]
Category Archives: General
Can you beat our Nanopore read error correction? We hope so!
Tagging of individual molecules has been used as an effective consensus error-correction strategy for Illumina data (Kivioja et al 2011, Burke et al 2016, Zhang et al 2016) and the principle is similar to the circular consensus sequencing strategy used to generate consensus reads with error rate of < 1 % on the PacBio (Travers et […]
Analysing amplicon data, how easy does it get?
Ever done amplicon DNA sequencing of the 16S rRNA gene to identify microbes? If so, then you must know about the challenge of analysing such complex data easily. My name is Kasper, and I am currently a master student here at the Albertsens lab. When I first learned how DNA is sequenced today, I was astonished by the rapid […]
Promethion configuration test and “reboxing”
Following our PromethION unboxing event we have finally managed to connect the machine to our University network and pass the configuration run. The configuration test demonstrates that the network can handle the data produced and transfer it fast enough to a network storage solution. Got everything up and running for @nanopore #PromethION CTC run thx to […]
How to spend 10.000.000 DKK (1.3 mill. EUR)
Recently, I was one of 16 recipients of a 10 mill. DKK grant (1.3 mill. EUR) from the VILLUM foundation under their Young Investigator Program (YIP). The program is unique in Denmark and offers young scientists an opportunity to build a research group on their own terms. The foundation is working on the premise of the founder […]
Following our experiences with DNA sequencing using the MinION since 2014 as a part of the Minion (early) Access Programme (MAP), and their developers programme we applied for a spot on the PromethION Early Access Programme (PEAP) back in May 2015. The MinION was the mindblowing DNA sequencer that allows you to do long read (no […]
Welcome to the Albertsen Lab!
The purpose of this blog is to give a glimpse into the Albertsen Lab. The blog will be managed by all lab members that will blog on what they are currently working on or what they find exciting. A few key-words on upcoming blog themes:Oxford Nanopore PromethIon unboxing; 3D printing DNA extraction devices; Full length […]