Genome assembly has been a big challenge since the first methods for analysing DNA sequences saw the light of day. The challenge is primarily a result of our inability to read long fragments of DNA. With an explosion in the market for long read sequencing technologies (pacbio, nanopore, 10X, longas, etc) there is now hope […]
Category Archives: Genomics
Why is it important to remove short molecules?
In the age of short read sequencing you could treat the cells with very harsh physical disruption as there was little chance that your DNA extraction could shear your DNA to shorter fragments than the machines could sequence. However, with the emerging long read sequencers short fragments are suddenly no fun and people have started […]
What is a good genome assembly?
With short reads you will often get fragmented but high single base accuracy assemblies and with long error prone reads you can get a single contig assembly with just a few ultra long reads but with a lot of errors due to the lower read quality. Some of these errors can be fixed by adding more coverage […]